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Sympathetic Nervous Activity is Involved in the Anti-Inflammatory Effects by Electroacupuncture Stimulation
전침자극 매개성 항염증 반응에 대한 교감신경의 작용연구
Korean J Acupunct 2019;36:162-170
Published online September 27, 2019;  https://doi.org/10.14406/acu.2019.015
© 2019 Society for Meridian and Acupoint.

Byung Gon Jo* , Nam Hoon Kim* , Uk Namgung
조병곤*ㆍ김남훈*ㆍ남궁욱

Department of Korean Medicine, Daejeon University
대전대학교 한의학과
Correspondence to: Uk Namgung
Department of Korean Medicine, Daejeon University, Daejeon 34520, Korea
Tel: +82-42-280-2614, Fax: +82-42-274-2600, E-mail: unamgung@dju.kr
*These authors contributed equally to this work.
Received August 14, 2019; Revised September 17, 2019; Accepted September 17, 2019.
This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objectives : Increasing evidence suggests that parasympathetic vagus nerve activity plays a role in modulating acupunctureinduced anti-inflammatory reaction, but the function of sympathetic nerve is not known. Here, we investigated whether splanchnic sympathetic nerve activity was involved in the regulation of splenic expression of TNF-α mRNA by electroacupuncture (EA) in LPS-injected animals.
Methods : DiI was injected into the stomach or celiac ganglion (CG) for retrograde labeling of the target tissues. EA was given at ST36 and the electrical stimulation on the sciatic nerve in LPS-injected mice. c-Fos signals in the tissues were analyzed by immunofluorescence staining, and TNF-α mRNA was analyzed by real-time PCR.
Results : Application of EA at ST36 or electrical stimulation on the sciatic nerve induced c-Fos expression in neurons of the spinal cord and celiac ganglion (CG). Then, the vagotomy reduced c-Fos levels in CG neurons but not in the spinal cord in animals given EA. Expression of TNF-α mRNA which was induced in the spleen after LPS was significantly inhibited by EA, then the vagotomy elevated TNF-α mRNA level similar to that in LPS-injected animals. Splanchnectomy in animals given LPS and EA also increased TNF-α mRNA though it was less effective than vagotomy.
Conclusions : Our data suggest that EA delivered to the spleen via the splanchnic sympathetic nerve may be involved in attenuating splenic inflammatory responses in LPS-injected animals.
Keywords: sympathetic nervous system, anti-inflammation, electroacupuncture, TNF-α, ST36


September 2019, 36 (3)
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