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The Effect of Woogakseungmatang Extract on NO Production in LPS- Stimulated RAW 264.7 Cells
우각승마탕이 LPS로 유도된  RAW 264.7 세포에서 NO 생산에 미치는 영향
Korean J Acupunct 2018;35:166-173
Published online December 27, 2018;  https://doi.org/10.14406/acu.2018.021
© 2018 Society for Meridian and Acupoint.

Na Young Jo
조나영

Department of Acupuncture & Moxibustion Medicine, Je-Cheon Hospital of Traditional Korean Medicine, Semyung University
세명대학교 제천한방병원 침구의학과
Correspondence to: Na Young Jo
Department of Acupuncture & Moxibustion Medicine, Je-Cheon Hospital of Traditional Korean Medicine, Semyung University, Semyung-ro 66, sinwoul-dong, Jecheon 27136, Korea
Tel: +82-43-649-1825, Fax: +82-43-645-1382, E-mail: cswcny2@hanmail.net
Received August 27, 2018; Revised September 20, 2018; Accepted September 20, 2018.
This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objectives : Woogakseungmatang is a prescription medication mainly used to treat facial paralysis in Korean medicine. The purpose of this study is to investigate the effects of Woogakseungmatang on anti-inflammation and anti-oxidation. 
Methods : Woogakseungmatang was extracted using hot water. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide(MTT) method; nitric oxide(NO) production and Prostaglandin E2(PGE2) production in RAW cells treated with Woogakseungmatang were investigated; and the cytokine changes associated with inflammation were examined. The antioxidant capacity of Woogakseungmatang was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method.
Results : RAW cells treated with Woogakseungmatang showed 90% cell viability at a 100-μg/ml concentration. NO production was decreased by 15% at a 100-μg/ml concentration. PGE2 production was decreased by 18% at a 100-μg/ml concentration. Interleukin 1β(IL-1β), interleukin 6(IL-6), and tumor necrosis factor-α(TNF-α) were significantly reduced at 100 μg/ml compared with those in the control group. The DPPH free radical scavenging capability was more than 50% at 100 μg/ml. 
Conclusions : Woogakseungmatang showed only a slight anti - inflammatory effect at 100 μg/ml and it was difficult to confirm the concentration-dependent anti-inflammatory effect. Therefore, this study means to confirm the potential anti-inflammatory effects of Woogakseungmatang. Based on this research, more systematic and diverse studies should be conducted.
Keywords: Woogakseungmatang, Seogakseungmatang, Anti-inflammation, Antioxidant, Macrophage, Korean Medicine


December 2018, 35 (4)
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