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Effects of the Pharmacopuncture with MOK on Immune Regulation by Th1/Th2 Cytokines in L-Thyroxine-Induced Hyperthyroid Rats
L-thyroxine으로 유발된 갑상선기능항진증 랫트에서 MOK 약침의 Th1/Th2 사이토카인 조절에 의한 면역조절 효과
Korean J Acupunct 2018;35:91-97
Published online June 27, 2018;
© 2018 Society for Meridian and Acupoint.

Ji Hye Hwang1 , Hyo Won Jung2

1Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University, 2Korean Medicine R&D Center, College of Korean Medicine, Dongguk University
1가천대학교 한의과대학 침구의학과, 2동국대학교 한의과대학
Correspondence to: Hyo Won Jung
Korean Medicine R&D Center, College of Korean Medicine, Dongguk University, Dongdaero 123, Gyeongju 38066, Korea
Tel: +82-54-770-2367, Fax: +82-54-770-2647, E-mail:
Received April 18, 2018; Revised June 9, 2018; Accepted June 9, 2018.
This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives : This study was performed to investigate immune regulatory effects of the pharmacopuncture with MOK on hyperthyroid rats. Methods : The experimental hyperthyroidism was prepared by the intraperitoneal injection of L-thyroxin (LT4, 0.5 mg/kg) once daily for 2 weeks in Sprague-Dawley(SD) rats. The pharmacopuncture with MOK extrac (MOK pharmacopuncture) at doses of 0.3 or 3 mg/kg was injected on acupuncture points in the thyroid glands of hyperthyroid rats once a day for 2 weeks. Propylthiouracil(PTU, 10 mg/kg) as a reference group was subcutaneously injected into the dorsal neck. We measured the levels of IFN-γ and IL-4 in the sera of rats using enzyme-linked immunosorbant assay(ELISA) and determined the expression of IFN-γ, IL-4, IL-10, and Foxp3 in spleen tissues by reverse transcriptase-polymerase chain reaction(RT-PCR). Results : The treatment of MOK pharmacopuncture in hyperthyroid rats significantly decreased the serum levels of Th1 cytokine, IFN-γ(p<0.01 for MOK 0.3 mg/kg, p<0.05 for MOK 3 mg/kg, and p<0.05 for PTU) and significantly increased the levels of Th2 cytokine, IL-4(p<0.05 for MOK 0.3 mg/kg, p<0.001 for MOK 3 mg/kg, and p<0.05 for PTU) compared to control group. Also, the MOK pharmacopuncture significantly increased IL-4 expression(p<0.05 for MOK 3 mg/kg, and p<0.05 for PTU), IL-10(p<0.05 for MOK 3 mg/kg, and p<0.01 for PTU), and Foxp3(p<0.01 for MOK 0.3 mg/kg, p<0.05 for MOK 3 mg/kg and p<0.01 for PTU) in spleen tissues of hyperthyroid rats compared to control group. Conclusions : Our results suggest that MOK pharmacopuncture can help to ameliorate the pathological progression of hyperthyroidism by regulation of the Th1/Th2 imbalance.
Keywords: pharmacopuncture, hyperthyroidism, immune regulation, Th1/Th2 cytokines, MOK

June 2018, 35 (2)
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