Fig. 4. The effect of PLP on the phosphorylation of MAPKs in HaCaT cell. To analyze the effect of PLP on MAPKs signaling pathway, HaCaT cells were were treated with PLP 100 μg/ml for 1 h and then treated with TNF-α/IFN-γ (each 10 ng/ml) for 5, 15 and 30 min. The phospho-rylation of MAPKs were analyzed in whole protein lysates. (A) Total MAPKs (t-ERK, t-JNK and t-p38) and phosphorylated MAPKs (p-ERK, p-JNK and p-p38) were determined by western blot analysis. (B) p-ERK, (C) p-JNK and (D) p-p38 were quantified in each total form. All experiments were conducted at least 3 times. All data represent the means ± SEM. ^#p<0.05 and ^##p< 0.01 versus non-treated group; *p< 0.05 versus only TNF-α/IFN-γ- treated group.

Korean J Acupunct 2022;39:43-53 https://doi.org/10.14406/acu.2022.007

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