Fig. 2. The effect of PLP on the expression of pro-inflammatory cytokines in HaCaT cells. To analyze the effect of PLP on cytokine expression, HaCaT cells were treated with PLP (12.5, 25, 50 and 100 μg/ml) for 1 h and then treated with TNF-α/IFN-γ (each 10 ng/ml) for 24 h. The concen-tration of (A) GM-CSF, (B) MCP-1, (C) IL-1β and (D) TNF-α in the cell culture medium was determined by ELISA. All experiments were con-ducted at least 3 times. All data represent the means ± SEM. ^#p< 0.05 and ^##p<0.01 versus non- treated group; *p<0.05 and **p< 0.01 versus only TNF-α/IFN-γ- treated group.

Korean J Acupunct 2022;39:43-53 https://doi.org/10.14406/acu.2022.007

© 2022 Korean J Acupunct