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Primer Sequence for RT-PCR Analysis
| Name | Sequence | Base pair | Tm (℃) | Cycle | Accession No. |
|---|---|---|---|---|---|
| TARC/CCL17 | F-ACTGCTCCAGGGATGCCATCGTTTTT R-ACAAGGGGATGGGATCTCCCTCACTG |
270 | 57.5 | 44 | NM_002987.3 |
| IL-6 | F-GATGGCTGAAAAAGATGGATGC R-TGGTTGGGTCAGGGGTGGTT |
230 | 59 | 45 | NM_000600.4 |
| CXCL8/IL-8 | F-ACATGACTTCCAAGCTGGCCG R-TTTATGAATTCTCAGCCCTC |
303 | 57.5 | 44 | NM_000584.4 |
| GAPDH | F-CGTCTAGAAAAACCTGCCAA R-TGAAGTCAAAGGAGACCACC |
52 | 50 | 30 | NM_001256799.3 |
TARC : thymus and activation-regulated chemokine, IL-6 : interlukin-6, IL-8 : interlukin-8, GAPDH : glyceraldehyde-3-phosphate dehydrogenase.