Korean Journal of Acupuncture

Fig. 6.

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Fig. 6. Effects of EELJ on the expression of Bax and Bcl-2 in glutamate-treated hippocampal HT22 cells. Cells were pre-treated with or without 10 μg/ml EELJ for 12 h, and then treated with or without 5 mM glutamate for 24 h. (A) The equal amounts of proteins were separated by SDS-polyacrylamide gel electrophoresis and subjected to Western blot analysis. Actin was used as an internal control, and the proteins were visualized using an ECL detection system. (B) The expression of each protein was indicated as a fold change relative to the control. Quantitative analysis of mean pixel density was performed using the ImageJ software. The results are expressed as the mean±SD obtained from three independent experiments (*p< 0.05 compared with the control group; #p<0.05 compared with the glutamate-treated group).
Korean J Acupunct 2021;38:32-42 https://doi.org/10.14406/acu.2021.002
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