Korean Journal of Acupuncture

Fig. 4.

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Fig. 4. Inhibitory effect of EELJ on glutamate-induced nuclear morphological changes and increase of sub-G1 cells in hippo-campal HT22 cells. Cells were pre-treated with or without 10 μg/ml EELJ for 12 h before treatment with 5 mM glutamate for 24 h. (A) The cells were stained with DAPI solution and stained nuclei were observed using a fluorescence microscope (original magnification, ×200). Each image is representative of at least three independent experiments. Scale bar=200 μM. (B and C) The cells were stained with PI solution for flow cytometry analysis. (B) PI fluorescence intensity was detected to measure the frequency of cells in each phase of the cell cycle using a flow cytometer. (C) Sub-G1% was calculated as the percentage of the number of cells in the sub-G1 population relative to the number of total cells. The results are expressed as the mean±SD obtained from three independent experiments (*p<0.05 compared with the control group; #p<0.05 compared with the glutamate-treated group).
Korean J Acupunct 2021;38:32-42 https://doi.org/10.14406/acu.2021.002
© 2021 Korean J Acupunct