Korean Journal of Acupuncture

Fig. 4.

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Fig. 4.

Effect of EA on CCL2 expression and microglial activation in neuropathic animals. (A) Western blot analysis of L5/6 ipsilateral spinal cord was performed on day 8 after SNL surgery. CCL2 expression has been decreased in ACU animals compared to ANE animals. (B) The ACU animals showed increased expression of TNFα compared to ANE animals. Data are presented as Mean±SEM of the ratio of area of CCL2 to area of actin. *indicates values that are significantly different from the ANE animals. Immunofluorescence analysis of L5/6 ipsilateral spinal cord was performed on day 8 after SNL surgery. (C) CCL2 expression in ANE (a), NAP (d), ACU (g) groups. The activation of microglia (Iba-1; microglial marker) was shown in ANE (b), NAP (e), ACU (h) animals. The merged cells were shown in ANE (c), NAP (f), ACU (i) animals. (D) ACU animals showed decreased number of both microglial cells and TNFα expression with a statistical difference to the ANE animals. Data are presented as MEAN±SEM of number of Iba-1 positive cells (a), number of CCL2 immunoreactive spots (b), number of merged cells (c). * and # indicate values that are significantly different from the ANE and NAP groups respectively (p<0.05). Scale bar is 100 μm. Arrow head indicates the co-localized cells.

Korean J Acupunct 2019;36:264-73 https://doi.org/10.14406/acu.2019.028
© 2019 Korean J Acupunct