Korean Journal of Acupuncture

Fig. 3.

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Fig. 3.

Expression of CCL2 and microglia in Naïve and SNL animals. (A) Western blot analysis of L5/6 ipsilateral spinal cord was performed on day 8 after SNL surgery. CCL2 expression has been increased in SNL animals compared to naïve animals. (B) The SNL animals showed increased expression of CCL2 compared to naïve animals. Data are presented as Mean±SEM of the ratio of area of CCL2 to area of actin. *indicates values that are significantly different from the Naïve group. Immunofluorescence analysis of L5/ 6 ipsilateral spinal cord was performed on day 8 after SNL surgery. (C) CCL2 expression has been statistically increased in SNL animals compared to naïve animals (a and d). The activation of microglia (Iba-1; microglial marker) was more evident in the SNL animals when compared to naïve animals (b and e). The merged cells were also increased in SNL animals compared to Naïve animals (c and f). (D) SNL group showed increased number of both microglial cells and CCL2 expression with a statistical difference to the Naïve group. Data are presented as MEAN±SEM of number of Iba-1 positive cells (a), number of CCL2 immunoreactive spots (b), number of merged cells (c). *indicates values that are significantly different from the Naïve group (p<0.05). Scale bar is 100 μm. Arrow head indicates the co-localized cells.

Korean J Acupunct 2019;36:264-73 https://doi.org/10.14406/acu.2019.028
© 2019 Korean J Acupunct